Journal: Cancers

Article Title: Multiparametric Characterization of the DSL-6A/C1 Pancreatic Cancer Model in Rats

doi: 10.3390/cancers16081535

Figure Lengend Snippet: List of antibodies used for the immunological analysis of the DSL-6A/C1 tumors.

Article Snippet: CD11a , PE , REA596 , Human cell line , Miltenyi , 130-109-171.

Techniques:

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 1. LFA1 was overexpressed induced by Ang II in the aortic tissues. (A) The mRNA level of LFA1 in both the saline and Ang II groups (n = 6); (B) The protein level of LFA1 in both the saline and Ang II groups (n = 6); (C) The protein level of LFA1 in both the control and SHR groups (n = 6); (D) The protein level of LFA1 in both the saline and DOCA groups (n = 6); (E) The area of positive LFA1 cells detected by IHC in both the saline and Ang II groups(n = 8); (F) Representative images of immunofluorescence staining (LFA1, Vimentin, α-SMA and CD31). (A indicates adventitia, E, endothelium, M, media). Statistical analysis was performed with 2- tailed unpaired Student t-test. A significance level of **P < 0.01 and ***P < 0.001 was considered statistically significant.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques: Saline, Control, Immunofluorescence, Staining

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 2. Intervening in LFA1 can inhibit Ang II-induced hypertension and vascular remodeling. (A) The systolic blood pressure (SBP) was measured in each group at various time points(n = 8); (B)The heart rate was recorded in each group(n = 8); (C) Images of H&E staining were taken (left) and quantitative measurements of aortic wall thickness were obtained (right)(n = 8); (D) Images of Masson staining were captured (left) and quantitative measurements of the fibrosis area were obtained (right)(n = 8); (E) The mRNA expression levels of collagen I, collagen III, and α-SMA were measured(n = 6). GAPDH was used as an internal reference; (F) The protein expression levels of α-SMA were measured (n = 5). Statistical analysis was performed with two-way ANOVA (A) and one-way ANOVA (B–F). A sig nificance level of */#P < 0.05 and ***/###P < 0.001 was considered statistically significant.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques: Staining, Expressing

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 3. Neutralizing LFA1 can inhibit Ang II-induced macrophage infiltration and oxidative stress levels. (A) Representative images of immunofluorescence staining (CD68, left), and quantitative images of CD68-positive macrophages (right) (n = 8); (B) mRNA expression of IL-6, IL-1β, TNF-α (n = 6); (C) Representative images of DHE (left) and quantification of fluorescence intensity (right) (n = 8); (D) Representative images of p-ATM (left) and quantification of fluorescence intensity (right) (n = 8); (E) mRNA expression of NOX1, NOX2 and NOX4 (n = 6); (F) The protein expression levels of p-p65, p65, NOX4 and gapdh were measured (n = 5). Statistical analysis was performed with one-way ANOVA (A, B (IL-1β,IL-6), C-F) and Kruskal-Wallis test (B (TNF-α)). A significance level of */#P < 0.05, **/##P < 0.01 and ***/###P < 0.001 was considered statistically significant.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques: Immunofluorescence, Staining, Expressing, Fluorescence

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 4. Neutralizing LFA1 can improve Ang II-induced endothelial dysfunction. (A) endothelium-dependent relaxation in each group (n = 6); (B) endothelium- independent relaxation of SNP in each group; (C, D) The protein expression levels of p-eNOS, eNOS and gapdh were measured (n = 5). Statistical analysis was performed with two-way ANOVA (A, B) and one-way ANOVA (C, D). A significance level of */#P < 0.05 and **/##P < 0.01 was considered statistically significant.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques: Expressing

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 5. LFA1 neutralizing antibody inhibits Ang II-induced HUVECs DNA damage and macrophage migration. (A) Immunofluorescence staining of p-ATM in HUVECs (n = 6); (B) quantification of p-ATM-positive foci (n = 6); (C) quantification of p-ATM foci/HUVECs (n = 6); (D) Immunofluorescence staining of γ-H2AX in HUVECs (n = 6); (E) quantification of γ-H2AX-positive foci (n = 6); (F) quantification of γ-H2AX foci/HUVECs (n = 6); (G) DAPI staining was used to visualize the mac rophages in each group (n = 6); (H) The number of migrated cells was analyzed in each group (n = 6). Statistical analysis was performed with one-way ANOVA (A–H). A significance level of ****P < 0.0001 was considered statistically significant.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques: Migration, Immunofluorescence, Staining

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 6. Ori inhibited Hypertension, Vascular Fibrosis, and Inflammation by Ang II-induced hypertension. (A–B) The molecular docking between CAR and USP18. (C) SBP of each group at different times was detected (n = 8); (D) heart rate in each group (n = 8); (E) Representative images of H&E staining (left), and quantitative images of aortic wall thickness (right) (n = 8); (F) Representative images of Masson (left) and quantitative images of fibrosis area (right) (n = 8); (G) mRNA expression of collagen I and collagen III (n = 6); (H) The protein expression levels of LFA1, α-SMA and gapdh were measured (n = 5). (A: indicates adventitia, E: endothelium, M: media.) Statistical analysis was performed with two-way ANOVA (C) and one-way ANOVA (C, D). A significance level of */#P < 0.05 and ***/###P < 0.001 was considered statistically significant.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques: Staining, Expressing

Journal: European journal of pharmacology

Article Title: New LFA-1 inhibitor Orientin reduces angiotensin II-induced vascular remodeling.

doi: 10.1016/j.ejphar.2025.177426

Figure Lengend Snippet: Fig. 8. A working model of Orientin and LFA1-mediated in the regulation of Ang II-induced vascular remodeling.

Article Snippet: Anti-LFA1 neutralizing antibody (M17/4, BE0006, Bioxcell, 100 μg, i.p. injection) or IgG control was applied 3 consecutive days before Ang II infusion and then every 2 days after surgery(Rothhammer et al., 2011).

Techniques:

Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Cytometry

Journal: Cancer Cell

Article Title: Multimodal stimulation screens reveal unique and shared genes limiting T cell fitness

doi: 10.1016/j.ccell.2024.02.016

Figure Lengend Snippet: Loss of Icam1 -mediated homotypic T cell interactions amplifies CD8 T cell expansion and improves effector functions shortly after TCR stimulation (A) Overlapping genes from top 50 hits from each screen, genes are ranked by average effect size. (B) STRING protein-protein interaction analysis of shared targets from the two boxes in (A) (32 genes). Interactions include direct (physical) and indirect (functional) associations. (C) Kaplan-Meier OS curves of patients receiving TIL therapy , (Besser cohort) with high or low ICAM1 expression in TIL products. Significance calculated with regular log rank test. (D) Microscopy images of indicated T cells 24 h after CD3 stimulation (n = 7 biological replicates). (E) Viable Ctrl and Icam1 -KO T cell counts under indicated stimulation conditions as in the screens, analyzed with Mann-Whitney test (n = 4–7 biological replicates). (F) Viable B16.OVA cells after 4 days co-culture with indicated T cells, analyzed with Mann-Whitney test (n = 4 biological replicates). (G) Transcriptomic profiling heatmap of indicated T cells with or without 24 h-CD3 stimulation, showing significantly (p value <0.001) differentially expressed genes ( Table S3 ). (H) Proteomic STRING enrichment analysis of differentially expressed proteins comparing Icam1 -KO with Ctrl T cells after 24 h-CD3 stimulation, showing top enriched GO biological process (ranked by enrichment strength (Log 10 (observed/expected)) with FDR < 0.1 ( Table S3 ). (I) Flow cytometry-based cytokine bead array showing cytokines released in the culture medium of indicated T cells after 24 h-CD3 stimulation, analyzed with Mann-Whitney test (n = 7 biological replicates). (J) Viable cell counts of Ctrl or Icam1 -KO T cells ectopically expressing wild type (wt) or mutated ICAM1 (lacking the intracellular domain, dcyt). Cell count was assessed 4 days after CD3 stimulation; analyzed with one-way ANOVA with Holm-Sidak’s multiple comparisons test (n = 3 biological replicates). OE: overexpression. (K) Viable cell counts of Ctrl or ICAM1/2/3 -KO human T cells 1 week after 24 h-CD3 stimulation, analyzed with Mann-Whitney test (n = 5 biological replicates). (L) Viable cell counts of human CD8 cells 1week after CD3 stimulation with or without CD54 (ICAM1) or CD11a (LFA1) blocking antibodies, analyzed with Kruskal-Wallis test with Dunn’s post-hoc test (n = 3 biological replicates). Error bars indicate SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: T cell reactivation was performed in the presence of CD11a antibody (1 μg/ml, clone R7-1, BioXCell), CD54 (1 μg/ml, clone R6-5-D6, BioXCell) antibody, CD178/FasL (10 μg/ml, Clone MFL3 (RUO), BD Pharmingen) or respective isotype controls (1 μg/ml, mouse IgG2a, clone C1.18.4 and mouse IgG1, clone MOPC-21, both BioXCell; 10 μg/ml, Armenian Hamster IgG1, κ, BD Pharmingen).

Techniques: Functional Assay, Expressing, Microscopy, MANN-WHITNEY, Co-Culture Assay, Flow Cytometry, Cell Counting, Over Expression, Blocking Assay

Journal: Cancer Cell

Article Title: Multimodal stimulation screens reveal unique and shared genes limiting T cell fitness

doi: 10.1016/j.ccell.2024.02.016

Figure Lengend Snippet:

Article Snippet: T cell reactivation was performed in the presence of CD11a antibody (1 μg/ml, clone R7-1, BioXCell), CD54 (1 μg/ml, clone R6-5-D6, BioXCell) antibody, CD178/FasL (10 μg/ml, Clone MFL3 (RUO), BD Pharmingen) or respective isotype controls (1 μg/ml, mouse IgG2a, clone C1.18.4 and mouse IgG1, clone MOPC-21, both BioXCell; 10 μg/ml, Armenian Hamster IgG1, κ, BD Pharmingen).

Techniques: Purification, Blocking Assay, Control, Functional Assay, Recombinant, Staining, Cell Culture, Isolation, Binding Assay, CRISPR, Sequencing, RNA Sequencing, Software

Journal: Immunity

Article Title: Development of Tbet- and CD11c-expressing B cells in a viral infection requires T follicular helper cells outside of germinal centers

doi: 10.1016/j.immuni.2022.01.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: InVivoMAb anti-mouse LFA-1α , Bio X Cell , Cat# BE0006; RRID:AB_1107578.

Techniques: Plasmid Preparation, Virus, Recombinant, Irradiation, Staining, Cell Isolation, Sequencing, Oligo Synthesis, Software, Imaging

Journal: Channels

Article Title: Cone dystrophy and ectopic synaptogenesis in a Cacna1f loss of function model of congenital stationary night blindness (CSNB2A)

doi: 10.1080/19336950.2017.1401688

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: PKCα , Mouse , Purified Mouse Monoclonal IgG2a , 1:1000 , Cedarlane Labs, Burlington, ON , purified bovine brain PKCα (Clone MC5) , [ ] .

Techniques: Affinity Purification, CRAfT Assay, Purification, Recombinant